GLASGOW MEDIUM

DEFINITION: It is a mixture of salts, amino acids, vitamins and other components that are essential for cellular growth.

ACTION: Acts like a nutritive solution in cell culture.

PRODUCT SPECIFICATIONS: It is applied for human and other animal cell culture. There are two presentations: powder and liquid. The powder medium is stable for more than 24 months if well locked in freezer, promoting constant results at long term. GLASGOW medium is modified from the BME EAGLE by the addition of 10% of Tryptose Phosphate*, double of amino acids and vitamins and also 4.5g of glucose anhydrous.

ADVANTAGES: Tested in at least three different cellular lineage and also in lymphocyte culture. pH and osmolality are always determined. Each lot is only discharged if within physical, chemical and biological pattern, and also within those related to cellular growth.

PHYSICAL-CHEMICAL CHARACTERISTICS:

-Appearance: yellow-rosy homogeneous powder
-Particles size: approximately 20 microns
-Solubility: Limpid solution at concentration to be used (1X)
-pH at 25°C (without NaHCO3): 6.0 + 0.3
-pH at 25°C (with NaHCO3): 7.6 + 0.3
-Osmolality (without NaHCO3): 260 ± 5%
-Osmolality (with NaHCO3): 310 ± 5%

STORAGE: Store the powder or the final solution at 2 to 6°C. If these conditions are not maintained alterations may be observed, such as:

1. Change colour
2. Grainings
3. Insolubility
4. Alteration of pH
5. Incapacity to maintain its integrity under normal conditions.

PREPARATION: The GLASGOW powder is extremely hygroscopic and must not be exposure to the environment. Preparations with higher concentrations are not recommended due to the possibility of precipitate formation.

1. Measure out tridistillate water to approximately 90% of desired total volume of medium (15-20°C).
2. Add the powder and mix gently into equilibrium. DO NOT HEAT THE WATER.
3. After the powder is completely dissolved, add 1.2 g of sodium bicarbonate 5.6% for one liter.
4. Add antibiotics or other components if desired.
5. If necessary, adjust pH with HCl 1N or NaOH 0.1N, or also CO2.
6. Add tridistillate water to the final volume and filter the medium into sterile container using a 0.22m membrane filter.

PRESENTATION: Powder: bottles with powder for 1, 10 and 50 liters.
Liquid: Bottle with 100 and 500 ml.
STORAGE: 2 to 6°C
SHELF LIFE: 12 months

*This component is not part of formulation and must be add later.

COMPONENTS OF GLASGOW MEDIUM

INORGANIC SALTS	mg/L	L-phenylalanine	33,00
CaCl22H2O	265,00	L-threonine	47,60
FeNI9H2O	0,10	L-thryptophan	8,00
MgSO4	97,67	L-tyrosine	52,19
KCl	400,00	L-valine	46,80
NaHCO3	2.750,00	VITAMINS	mg/L
NaCl	6.400	Choline chloride	2,00
NaH2PO4	107,80	Folic Acid	2,00
AMINO ACIDS	mg/L	Myo-inositol	3,60
L-arginine	42,00	Niacinamide	2,00
L-cystine 2HCl	31,29	D-Pantothenate acid	2,00
L-glutamine	292,00	Pyridoxal	2,00
L-Histidine HCl.H2O	21,00	Riboflavin	0,20
L-isoleucine	52,40	Thiamine	2,00
L-leucine	52,40	OTHER COMPONENTS	mg/L
L-lysine	73,10	glicose	4.500
L-methionine	15,00	Phenol red	16,00